Sylvia D. Taqryanka and Evelyn Komaratih and Yuyun Rindiastuti and Helen Susilowati and Nurita Tri Wijayanti and Fedik Abdul Rantam (2019) Anifibrosis Effect of Fibrin Glue on TGF-â and â-SMA Expression in Human Tenon Fibroblast, as Wound Healing Model After Trabeculectomy: An In Vitro Study. Biochemical and Cellular Archives, 19 (Supp-2). pp. 4777-4781. ISSN 09725075

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Abstract

ABSTRACT : Fibrin glue (FG), which has already been known for its vital function as tissue adhesive, has lately been found with another important role in reducing inflammation and fibrosis especially in patients with glaucoma, who have gone through trabeculectomy. This study aimed at investigating the capability of fibrin glue in reducing profibrotic factors such as TGF- β and α-SMAat the early stage of fibrosis after trabeculectomy. This study used Human Tenon Fibroblasts (HTFs), as the key effector of bleb fibrosis after trabeculectomy. These were isolated from tenon tissues in eyes with glaucoma. These HTFs were cultured and divided into 3 groups made up of FBS 2% control group, MMCp and FG groups. Then, after 7 days of culture, TGF-β and α-SMA expression were assessed by immunofluorescence staining and its intensity was measured using ImageJ software. The results between groups were analyzed using Kruskal-Wallis or Brown forsythe test followed by posthoc test with 95% confidence interval (p<0.05). FG successfully decreased the expressions of TGF-â and α-SMA, which has a key role in fibrosis with mean expression intensity of 93.68±3.96% for TGF- β and 142.97±11.03% for α-SMA. The results showed that FG has a role in fibrosis inhibition considering the fact that it decreases profibrotic factors. However, MMC has the best antifibrotic effect among the experimental groups. FG plays a role as an antifibrotic agent in HTFs by reducing the profibrotic factors.

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