Evelyn Komaratih and Yuyun Rindiastuti and I Ketut Sudiana and Cita Rosita Sigit Prakoeswa and Fedik Abdul Rantam (2019) Protein Profile in Self-Made Fibrin Glue as Promising Biomaterial for Wound Healing Modulation After Trabeculectomy. Biochemical and Cellular Archives, 19 (Supp-2). pp. 4845-4850. ISSN 09725075

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Abstract

ABSTRACT : Subconjunctival fibrosis is the main cause of failure in trabeculectomy. Application of fibrin glue following conventional trabeculectomy is hypothesized to increase its surgical success rate. This study aimed to investigate the protein profile in self-made fibrin glue which could possibly contribute to wound healing modulation after trabeculectomy. Peripheral blood was obtained from a single donor using citrate phosphate dextrose adenine (CPDA-1) anticoagulant. Fibrin glue was generated from the mixture of fibrinogen and thrombin. Fibrinogen and thrombin were isolated from plasma pellet and calcium chloride (CaCl2) activated platelet rich plasma (PRP) components using gradient centrifugation alongside the single freezethaw cycle technique. Analysis of protein profile was conducted using liquid chromatography – mass spectrometry (LC – MS). Protein identification was obtained from the Universal Protein knowledge base (UniProt). Liquid chromatography – mass spectrometry analysis revealed 139 proteins in the self-made fibrin glue. There were 26 unique proteins related to the wound healing process, including growth factor related proteins, cell adhesion proteins, and platelet related proteins. There were 30 proteins corresponding to extracellular matrix remodeling which consists of 15 proteolysis activation related proteins, 9 proteolysis regulation related proteins, and 6 negative regulation of proteolysis related proteins. Moreover, as it is a biomaterial, our self-made fibrin glue could act as a biological spacer to facilitate aqueous outflow, and its degradation time of 7-14 days could also be taken advantage of. Self-made fibrin glue contains unique proteins which can contribute to wound healing modulation after trabeculectomy.

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