Debora Poerwantoro (2021) Aktivitas Asetilkolinesterase Inhibitor Dari Subfraksi N- Butanol Akar Pule Pandak (Rauvolfia Serpentina (L.) Bth.Ex Kurz). Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Rauvolfia serpentina is a plant that contains many alkaloid compounds. Some of Alzheimer's disease drugs derive from alkaloid compounds. The n-butanol fraction of R. serpentina root has the potential to inhibit acetylcholinesterase (AChE) with IC50 of 5.99 μg/mL. n-butanol fraction was fractionated using the Vacuum Liquid Chromatography (VLC) method with a gradient elution of dichloromethane and methanol which was arranged in 13 compositions in order increasing polarity. All n-butanol subfractions obtained from the VLC method were tested for its inhibitory activities against AChE by using Elmann's method. Samples with percentage inhibition ≥ 80% were followed by IC50 determination. From 13 samples, 4 subfractions showed percentage inhibition ≥ 80%, i.e. subfractions 4 to 7. The results of the study showed that the IC50 value of subfractions 4 to 7 are between 4.87 to 47.22 μg/mL. From these results, it can be seen that subfraction 5 has the strongest AChE inhibitor activity among the eight subfractions with IC50 value of 4.87 ± 0.45 μg/mL and subfraction 7 has the weakest AChE inhibitor activity with IC50 value of 47.22 ± 1.08 μg/mL. The TLC screening of subfractions 4-7 showed orange colour which indicated the presence of alkaloid. Based on these results, it can be concluded that the VLC subfractions of R. serpentina exhibited potent AChE inhibitory activity.

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