Abdullah, Muhammad Rizqy and Suhendro, Gatut and Deneska, Ria Sandy and Fauziah, Dyah and Notobroto, Hari Basuki and Lestari, Atina Yustisia and Rosati, Denisa (2021) Effect of limbal mesenchymal stem cell in form deprivation myopia animal model. Thesis thesis, ARSCB.

Text Tesis Rizqy ttd Prof Ir.pdf Download (17MB)

Abstract

Purpose: To investigate the capability of limbal mesenchymal stem cell (LMSC) in reducing myopia progression in form deprivation myopia animal model. Methods: A total of 24 rabbits (Oryctolagus cuniculus) divided into three equal groups(n=8) as follows: form deprivation myopia group (FDM), FDM group with treatment 1x10 5 in 0,1 ml LMSCs injection (FDM+LMSC), and control group. Refraction status was evaluated by streak retinoscopy and axial length was measured using a-scan biometry. The migration of LMSCs into posterior sclera were evaluated with red-fluorescent cell dye (PKH26). α-smooth muscle actin (α-SMA) expression and posterior scleral thickness were evaluated in a 6-week follow-up. Results: There were significant differences of α-SMA expression and scleral thickness between three groups (p<0,05). There were significant differences of scleral thickness and α-SMA expression between the control and the FDM with LMSCs group. Conclusion: LMSC has potential role for myopia control in form deprivation eye animal model by increasing α-SMA and sclera thickness. Keywords: Form deprivation myopia, limbal mesenchymal stem cell, α- SMA, myofibroblast, scleral thickness

Actions (login required)

View Item