Naimah Putri, - and Rahaju Ernawati, - and Suwarno, - and Jola Rahmahani, - and Fedik Abdul Rantam, - (2019) Fusion Protein of Aminoacid Mutations in Newcastle Disease Isolated from Swan Goose Caused Resistance to Infection. The Indian Veterinary Journal, 96 (10). pp. 53-55. ISSN 0973-9122, ISSN (ONLINE) : 0973-9130

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Abstract

Newcastle disease virus (NDV) cloacal swab were collected from unvaccinated swan goose and isolated by inoculation in Speci c Pathogen Free embryonated eggs. Haemagglutination and Haemagglutination inhibition tests were done to identify and the, samples were processed to one step Reverse Transcriptase-Polymerase Chain Reaction using primer that ampli ed sequences of fusion (F) gene. Deduced amino acid sequence of the cleavage site of fusion (F) protein revealed that all isolates had avirulent motifs. Amino acid mutations that occurred were analyzed to the same reference sequence. The result showed that amino acid mutations was not capable of changing the virulence of the virus Key words : Newcastle disease virus (NDV), amino acid, mutations and virulence

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