Utami Meilanie Putri, - and Maftuchah Rochmanti, - and Manik Retno Wahyunitisari, - and Rebekah Juniati Setiabudi, - (2021) The Antibacterial Effect of Ethanol Extract of Garlic (Allium sativum L.) on Methicillin Resistant Staphylococcus aureus (MRSA) In Vitro. Indian Journal of Forensic Medicine & Toxicology, 15 (2). pp. 3504-3509. ISSN 0973-9122

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Abstract

Objective: To determine the antibacterial activity of ethanol extract of garlic against Methicillin-Resistant Staphylococcus aureus. Method: Dilution test was performed on 9 different concentration of ethanol extract of garlic (1024, 512, 256, 128, 64, 32, 16, 8, 4 mg/mL) dissolved in DMSO 10% and MRSA suspension on Mueller Hinton broth medium to determine the Minimum Inhibitory Concentration. The Minimum Bactericidal Concentration was determined by the result of streaking of MRSA inoculation on the nutrient agar plate. Results: The observation through Mueller Hinton broth medium has shown turbidity both before and after incubation because of garlic ethanol extract color which is blackish-brown and thick, while in the nutrient agar medium, MRSA bacteria grew on the media with concentrations 4, 8, 16, 32, 64, & 128 mg/mL and unable to grow at concentrations of 256, 512, and 1024 mg/mL. Conclusion: The MIC of ethanol extract of garlic on MRSA could not be determined, and the MBC in this study was 256 mg/mL.

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