Aniek Setya Budiatin, - and Maria A. Gani, - and Samirah, - and Chrismawan Ardianto, - and Aulia M. Raharjanti, - and Indah Septiani, - and Ni Putu K. P. Putri, - and Junaidi Khotib, - (2021) Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect. International Journal of Biomaterials, 2021. pp. 1-7. ISSN 1687-8787

	Text (FULLTEXT) C-01 Artikel dan SJR.pdf Download (7MB)
	Text (KUALITAS KARIL) C-01_Kualitas_Karil_001.pdf Download (2MB)
	Text (SIMILARITY) C-01 Similarity_001.pdf Download (4MB)
	Text (KORESPONDENSI) C-01 Korespondensi_001.pdf Download (903kB)
	Text (FULLTEXT (SMR)) Bukti C-07 Artikel.pdf Download (11MB)
	Text (KUALTAS KARIL (SMR)) Bukti C-07 Kualitas Karil.pdf Download (379kB)
	Text (SIMILARITY (SMR)) Bukti C-07 Turnitin.pdf Download (4MB)
	Text (KORESPONDENSI (SMR)) Bukti C-07 Korespondensi.pdf Download (903kB)
	Text (FULLTEXT (CA)) Artikel C-22.pdf Download (12MB)
	Text (Kualitas Karil (CA)) (C-22) Kualitas Karil.pdf Download (585kB)
	Text (Similarity (CA)) Similarity C-22.pdf Download (4MB)

Abstract

Osteomyelitis is an infectious disease which is also a major complication of bone defects. This study aims to determine the effect of bovine hydroxyapatite-gelatin-based bone implants with gentamicin as an antibiotic (BHA-GEL-GEN implant) on the regeneration of bone defects in vivo. The BHA-GEL-GEN and BHA-GEL implants were made by direct compression. In vivo study was carried out with Wistar rats. The rats were divided into three groups: negative control, BHA-GEL implant, and BHA-GEL-GEN implants. The defect model used was the burr hole defect model with diameter 2.2 mm and 2 mm deep. After 2, 7, 14, and 28 days, the rats were sacrificed. Bone integrity was carried out using X-ray radiography. Radiological examination was performed using haematoxylin and eosin (HE) staining and immunohistochemical techniques with anti-vascular endothelial growth factor (VEGF) and anti-alkaline phosphatase (ALP) antibodies. Based on the radiograph, the implanted group had accelerated bone growth in the defect area. Semiquantitative data from HE staining showed that the implanted group had accelerated migration of osteoclasts, osteoblasts, and osteocytes in the defect area. The immunoreactive score showed that the BHA-GEL-GEN group had higher VEGF expression compared to two other groups. The three groups did not provide a significant difference in ALP expression. In conclusion, the BHA-GEL-GEN implant causes accelerated bone defects repair by accelerating tissue vascularity and does not interfere with the bone remodeling process. Therefore, the BHA-GEL-GEN implant is potentially a biomedical material for osteomyelitis therapy.

Actions (login required)

View Item