Eduardus Bimo Aksono Herupradoto, - (2017) Shark Species on Export Products from East Java and Bali by DNA Barcoding Based on Internal Transcribed Spacer-2 (ITS-2) Locus in Mitochondrial. The Veterinary Medicine International Conference. pp. 105-110. ISSN 2413-0877

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Abstract

DNA barcoding method is the mitochondrial marker for all animal species, and it isclaimed as distinguishing feature from one species to another Species identificationin shark products is often difficult to perform as they have morphological similaritieswith many other species and it is even more difficult as they are parts separated fromthe body for the storage. This research is aimed to know which species of sharksidentified in the export products from East Java and Bali by DNA barcoding method.The samples of sharks (meat, fins, skin and bones) used were 90 samples acquiredin Surabaya from the export products of East Java and Bali from 2015 to 2017. TheDNA barcoding method uses universal primer through nested PCR (Polymerase ChainReaction) process which is able to amplify the DNA until around 1,340 bp based onInternal Transcribed Spacer-2 (ITS-2) locus of mitochondria. Based on the result ofphylogenetic analysis and the classification list by IUCN, from 90 samples of sharksacquired from export products in East Java and Bali, species identified were: 1.11%Daenia sp categorized as NE (not evaluated); 4.44% Sphyrna zygaena categorized asNT (not Threatened); 3.33% Sphyrna lewini categorized as NT (not Threatened); 10%Rhizoprionodon taylori categorized as LC (least concern); 24% Charcarhinus brevipinnacategorized as NT (not Threatened); 2.22% Charcarhinus obscurus categorized as NT(not Threatened); 3.33% Charcarhinus falciformis categorized as LC (least concern);1.11% Charcarhinus plumbeus categorized as NT (not Threatened); 27.78% Charcarhinuslongimanus categorized as VU (vulnerable); 1.11% Neutrygon kuhlii categorized asNE (not evaluated); 1.11% Charcarhinus Taurus categorized as VU (vulnerable); 3.33%Rhizoprionodon longurio categorized as DD (data deficient); 1.11% Rhizoprionodonporosus categorized as DD (data deficient); 1.11% Eusphyra blochii categorized as NT(not Threatened); 4.44% Chiloscyllium griseum categorized as DD (data deficient);5.56% Rhizoprionodon oligolinx categorized as LC (least concern); 1.11% Prionace glaucacategorized as NT (not Threatened); 1.11% Rhizoprionodon lalandii categorized as DD(data deficient). Generally all species found in this research were special fish fromIndo-Australian archipelago and included in IUCN red list. The government policyto prohibit export of these species was the right decision to prevent the speciesextinction. Keywords: ITS-2; Shark; Export Products; Java and Bali

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