Luviana Kristianingtyas,, - and Mustofa Helmi Effendi, - and Wiwiek Tyasningsih, - and Fredy Kurniawan, - (2020) Genetic Identification of blactx-M Gene and blatem Gene on Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia Coli from Dogs. The Indian Veterinary Journal, 97 (1). pp. 17-21. ISSN 0019-6479 E-ISSN: 0974-9365

	Text (Journal) Karil Genetic Identification of blactx-M Gene.pdf Download (618kB)
	Text (Peer Review) Peer Review Genetic Identification of blactx-M Gene.pdf Download (2MB)
	Text (Turn It In) Turn It In Genetic Identification of blactx-M Gene.pdf Download (1MB)

Abstract

The purpose of this study was to identify the ESBL producing E. coli and the characteristics of the encoding genes. 84 rectal dog swabs from several animal clinics in Surabaya, Indonesia were taken for the study. All the 84 positive E. coli samples were con rmed by the Double Disc Synergy Test (DDST) method. Molecular identification of bla CTX-M and blaTEM ESBL encoding genes using mutiplex Polymerase Chain Reaction (PCR). ESBL-producing E. coli bacteria from rectal swabs were found to be 9.52% (8/84). The PCR results showed that the blaCTX-M gene consisted of 8 ESBL isolates and blaTEM gene as many as 1 ESBL isolate which have the potential as a reservoir in spreading antibiotic resistance to humans and animals. Key words : Escherichia coli, ESBL, blaCTX-M gene, blaTEM gene, Dogs

Actions (login required)

View Item