Zhafira Nur Shabrina, - and Ni Putu Mira Sumarta, - and Coen Pramono Danudiningrat, - (2018) A study of cytotoxicity and proliferation of Cosmos caudatus Kunt leaf extract in human gingival fibroblast culture. Dental Journal, 51 (4). pp. 180-185. ISSN 1978-3728

	Text (ARTIKEL) 14 A study of cytotoxicity-validasi.pdf Download (1MB)
	Text (SIMILARITY) 14 A study of cytotoxicity.pdf Download (1MB)
	Text (VALIDASI) 14_ A study of cytotoxicity and proliferation of Cosmos caudatus Kunt leaf extract in human gingival fibroblast culture.pdf Download (2MB)
	Text (KARIL) 21.pdf Download (964kB)

Abstract

Background: Post-extraction dental sockets clinically resolve within a period of 3-4 weeks. However, complete healing and bundling of gingival fibers may require several months. Medication is therefore required to accelerate the healing process. Cosmos caudatus, a local plant with antioxidant properties and high calcium content, has the potential to promote wound healing while also reportedly capable of strengthening bone. Previous studies have demonstrated the effectiveness of Cosmos caudatus as an alternative treatment for post-menopausal osteoporosis by investigating the dynamic and cellular parameters of bone histomorphometry. Purpose: The study aimed to examine the citotoxicity and proliferation of human gingival fibroblast cells culture after the application of Cosmos caudatus extract. Methods: Cultures of human gingival fibroblast cells with 5x104 cell density were divided into two groups and placed in a 30-well culture dish. The control group contained human gingival fibroblast cell culture without extract, while the experimental group consisted of human gingival fibroblast cells culture with extract. The concentrations of extract were 1200 μg/ml, 600 μg/ml, 300 μg/ml, 150 μg/ml, and 75 μg/ml. A toxicity test was conducted and the optimum concentration evaluated using an MTT assay, while fibroblast numbers on were calculated days 1 and 2 by means of a hemocytometer. Research data was analyzed using a one-way ANOVA test. Results: No toxicity was found. The optimum concentration was 600 μg/ml and fibroblast proliferation was significantly higher in the experimental group compared to the control group, p=0.002 (P<0.05). Conclusion: Cosmos caudatus leaf extract is non-toxic and increases the proliferation of human gingival fibroblast culture at an optimum concentration of 600 μg ml.

Actions (login required)

View Item