Resna, - and Aryati, - and Puspa Wardhani, - and Erwin Astha Triyono, - (2012) Nilai Diagnostik Anti Dengue IgA dan Ns1, serta IgM/IgG di Infeksi Virus Dengue. Indonesian Journal of Clinical Pathology and Medical Laboratory, 21 (1). pp. 82-89. ISSN 0854-4263

	Text (Artikel) 16 Artikel.pdf Download (474kB)
	Text (Peer Review) 16.pdf Download (1MB)
	Text (Similarity) 16 Similarity.pdf Download (2MB)

Abstract

The clinical manifestations of dengue virus infection are varied and thus a specific diagnostic examination is required. Usually antidengue IgM is often used, but the presence in the circulation is 3−8 months long. NS1 is sensitive in the detection of primary infection, whereas IgG is more better used in secondary infection. The examination of anti-dengue IgA as a new marker is estimated to be able to detect the acute primary and secondary infection, however the diagnostic value of anti-dengue IgA is not much well known for the Indonesian population. This study was done at the Tropical Infectious Disease Ward of Dr. Soetomo Hospital, Surabaya during February – April 2013. The samples consisted of 37 sera from patients infected by dengue virus and 37 sera from those non one (dengue virus infection patients). The NS1 serum, anti-dengue IgM and anti dengue IgG were examined by ELISA and anti-dengue IgA was examined by an indirect immunochromatography method using Assure@ Dengue IgA Rapid Test (MP Biomedicals Asia Pacific Pte Ltd). The diagnostic value was analyzed by 2x2 table with a confidence interval of (CI) 95%. The used gold standards were from the 1997th WHO criteria and one of the positive dengue serological tests by ELISA (NS1/anti dengue IgM/anti dengue IgG). AUC and anti-dengue IgA cut-off were determined by ROC curve. The Diagnostic value of anti-dengue IgA showed a sensitivity and specificity of 83.8% (67.3 to 93.2) and 81.1% (64.3 to 91.4). A positive predictive value of 81.6% (65.1 to 91.7) and a negative predictive value of 83.3% (66.5 to 93.0) was found. The positive likelihood ratio was 4.4 times (2.2 to 8.8) and negative likelihood ratio of only 0.2 times (0.09 to 0.42). The best cut off value of 0.2 was shown by the area under the curve of 83.5%. Based on this study, the diagnostic value of anti-dengue IgA had a good validity for the diagnosis of dengue virus infection.

Actions (login required)

View Item