R. A. Prastiya, - and Rimayanti, - and M. M. Munir, - and A. P. Nugroho, - (2021) The Protective Impacts of α-tocopherol Supplementation on the Semen Quality of Sapera Goat Preserved at 4⁰C. Tropical Animal Science Journal, 44 (3). pp. 261-266. ISSN p-ISSN 2615-787X e-ISSN 2615-790X

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Abstract

Supplementation of vitamin E (α-tocopherol) as an antioxidant into an extender is useful to main-tain sperm quality during the process of cryopreservation by protecting the sperms from reactive oxy-gen species (ROS). This study aims to evaluate the protective impacts of vitamin E supplementation on the motility, viability, and reducing the abnormality of sperms in Sapera goats. The sample of semen used in the present study was obtained from fresh semen and good-quality ejaculates of the Sapera goats collected by an artificial vagina. The groups involve one controlled treatment group, which con-tains semen and tris egg yolk (TEY) extender without vitamin E (P0), and three treatment groups that contain semen, TEY extender, and different doses of vitamin E, i.e., 1 mg/mL (P1), 2 mg/mL (P2), and 3 mg/mL (P3), respectively. Data obtained from the experiments were analyzed using Repeated Measures Multivariate Analysis (MANOVA). The results showed that TEY extenders added with vitamin E at a dose of 1 mg/mL of vitamin E (P1) produced the highest motility and viability with the lowest abnor-malities of sperm after 24th hour of cryopreservation. The lowest sperm motility and viability among the treatment groups was observed in the TEY extender added with vitamin E at a dose of 3 mg/mL (P3) at 96th hour after cryopreservation, which was almost the same as the control group without vitamin E (P0) that had the lowest motility and viability. In conclusion, the current study found that supplementation of vitamin E as an antioxidant at a dose of 1 mg/mL in TEY extender can preserve semen of the Sapera goats in refrigerator up to 96 h at 4°C and provides higher sperm motility and viability percentage, with a lower percentage of sperm abnormality. Keywords: vitamin E (α-tocopherol); extender; Sapera goat; sperm assessment; animal medicine

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