Laksmi Wulandari, - and Berliana Hamidah, - and Cennikon Pakpahan, - and Nevy Shinta Damayanti, - and Neneng Dewi Kurniati, - and Christophorus Oetama Adiatmaja, - and Monica Rizky Wigianita, - and Soedarsono, - and Dominicus Husada, - and Damayanti Tinduh, - and Cita Rosita Sigit Prakoeswa, - and Anang Endaryanto, - and Ni Nyoman Tri Puspaningsih, - and Yasuko Mori, - and Maria Inge Lusida, - and Kazufumi Shimizu, - and Delvac Oceandy, - (2021) Initial study on TMPRSS2 p.Val160Met genetic variant in COVID-19 patients. Human Genomics, 15 (29). pp. 1-9. ISSN 14739542

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Abstract

Background Coronavirus disease 2019 (COVID-19) is a global health problem that causes millions of deaths worldwide. The clinical manifestation of COVID-19 widely varies from asymptomatic infection to severe pneumonia and systemic inflammatory disease. It is thought that host genetic variability may affect the host’s response to the virus infection and thus cause severity of the disease. The SARS-CoV-2 virus requires interaction with its receptor complex in the host cells before infection. The transmembrane protease serine 2 (TMPRSS2) has been identified as one of the key molecules involved in SARS-CoV-2 virus receptor binding and cell invasion. Therefore, in this study, we investigated the correlation between a genetic variant within the human TMPRSS2 gene and COVID-19 severity and viral load. Results We genotyped 95 patients with COVID-19 hospitalised in Dr Soetomo General Hospital and Indrapura Field Hospital (Surabaya, Indonesia) for the TMPRSS2 p.Val160Met polymorphism. Polymorphism was detected using a TaqMan assay. We then analysed the association between the presence of the genetic variant and disease severity and viral load. We did not observe any correlation between the presence of TMPRSS2 genetic variant and the severity of the disease. However, we identified a significant association between the p.Val160Met polymorphism and the SARS-CoV-2 viral load, as estimated by the Ct value of the diagnostic nucleic acid amplification test. Furthermore, we observed a trend of association between the presence of the C allele and the mortality rate in patients with severe COVID-19. Conclusion Our data indicate a possible association between TMPRSS2 p.Val160Met polymorphism and SARS-CoV-2 infectivity and the outcome of COVID-19.

Item Type:	Article
Uncontrolled Keywords:	COVID-19, TMPRSS2, Polymorphism
Subjects:	R Medicine > R Medicine (General) R Medicine > RC Internal medicine
Divisions:	01. Fakultas Kedokteran > Ilmu Pulmonologi dan Ilmu kedokteran Respirasi
Creators:	Creators NIM Laksmi Wulandari, - NIDN8878210016 Berliana Hamidah, - UNSPECIFIED Cennikon Pakpahan, - UNSPECIFIED Nevy Shinta Damayanti, - UNSPECIFIED Neneng Dewi Kurniati, - UNSPECIFIED Christophorus Oetama Adiatmaja, - UNSPECIFIED Monica Rizky Wigianita, - UNSPECIFIED Soedarsono, - NIDN8849800016 Dominicus Husada, - NIDN8800010016 Damayanti Tinduh, - NIDN8889900016 Cita Rosita Sigit Prakoeswa, - NIDN8865610016 Anang Endaryanto, - NIDN8867700016 Ni Nyoman Tri Puspaningsih, - NIDN0015066305 Yasuko Mori, - UNSPECIFIED Maria Inge Lusida, - NIDN0017095807 Kazufumi Shimizu, - UNSPECIFIED Delvac Oceandy, - UNSPECIFIED
Depositing User:	arys fk
Date Deposited:	23 Oct 2022 23:02
Last Modified:	23 Oct 2022 23:02
URI:	http://repository.unair.ac.id/id/eprint/118343
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