Malika, - (2010) Uji Kadar Igg Serum Tikus Putih Yang Diaplikasi Dengan Enzim Glukanase Sebagai Kandidat Penghambat Pembentukan Plak Gigi. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Glucanase enzymes isolate of bacteria B4, B5 and B7 isolated from decayed wood soil and mud in vitro have proven effective in preventing dental plaque. Glucanase enzymes are a protein that have the potential to become antigen. The biocompability of these enzymes were tested immunologicaly viewed from immunohumoral response through increasing of IgG level in rat blood serum. Purpose. To identify body humoral immune response on glucanase enzymes exposure from isolate B4, B5, and B7 as candidate of dental plaque formation. Method To measure rat serum IgG level with Indirect ELISA method viewed from optical density (OD) value by using ELISA Reader in wavelength 1450 rn. Results. There were no significant dfference on IgG level increase between B4 and B5 groups where P = (.171 > a 0.05). Conclusion. Glucanase enzymes from bacteria isolates B4 and B5 as candidate of dental plaque inhibition were not providing humoral reaction on rat body by observing the increase of IgG level.

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