Fedik Abdul Rantam, - and Alexander Patera Nugraha, - and Ferdiansyah Mahyudin, - and Purwati, - and Candra Bumi, - and Helen Susilowati, - and Eryk Hendrianto, - and Dwikora Novembri Utomo, - and Heri Suroto, - and Christrijogo Sumartono Waloejo, - and Rosy Setiawati, - and Cita Rosita Prakoeswa, - and Diah Mira Indramaya, - (2020) A Potential Differentiation of Adipose and Hair Follicle-derived Mesenchymal Stem Cells to Generate Neurons Induced with EGF, FGF, PDGF and Forskolin. Researh Journal of Pharmacy and Technology, 13 (1). pp. 275-281. ISSN 0974360X

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Abstract

Human Adipose Derived Mesenchymal Stem Cells (HADMSCs) and Human Hair Follicle Derived Mesenchymal Stem Cells (HHFDMSCs) have attracted great interest because of their multilineage differentiation potential, self-renewal properties, and their possible use of cell and gene therapies. This present study to investigate the neurogenic differentiation ability of hADMSCs and hHFDMSCs induced by Epidermal Growth Factors (EGF), Fibroblast Growth Factor (FGF), Platelet Derived Growth Factor (PDGF) and Forskolin. This study was true experimental with longitudinal study design. The sample size determined with minimal sample size formula and it was randomly chosen. These studies employed an in vitro design for the expansion and proliferation of Mesenchymal Stem Cells (MSCs) and examined the heterogeneity of these cells using the markers CD105, CD90, OCT4, and SOX2. MSCs from adipose tissue and hair follicles were induced with EGF, FGF, PDGF and Forskolin to differentiate and generate neurons. The capacity of MSCs to generate neurons were verified using glial fibrillary acidic protein, nestin, and ß-tubulin III . The expression of neural markers and morphological changes in Mesenchymal stem cells from hADMSCs and hHFDMSCs were confirmed. hADMSCs and hHFDMSCs share a similar capacity to differentiate and generate neurons, which is beneficial for the development of neuronal restoration for future therapies for patients suffering from neurological diseases.

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