Herin Setianingsih, 090515538/M and Soetjipto, Soetjipto and I. Ketut Sudiana, I. Ketut and Widjiati, Widjiati and Guritno Suryokusumo, Guritno and Johanes Nugroho Eko Putranto, Johanes The effect of the endothelial nitric oxide synthase on hypoxia-induced factor-1 alpha level in a state of endothelial dysfunction after hyperbaric oxygen therapy. Drug Invention Today, 12 (11).

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Abstract

Abstract Aim: This research aims to know the effect of eNOS on hypoxia-induced factor-1 alpha (HIF-1α) levels in endothelial dysfunction after hyperbaric oxygen therapy (HBOT). Materials and Methods: The design of this study was experimental research; the study sample consisted of 30 Sprague Dawley (Rattus norvegicus) white rats and divided into three groups p1, p2, and p3; p1 rats with a standard diet, p2 rats with a high-cholesterol diet, and p3 rats with a high-cholesterol diet continued HBOT at 2.4 ATA with 98% O2 for three sessions with a duration of 30 min/session, and air brakes for 5 min between each session for 10 consecutive days. eNOS and HIF-1α were examined using the enzyme-linked immunosorbent assay method. Results: From the results of testing the significance of the regression model, the results showed that there was a relation of HIF-1α on eNOS levels (P = 0.009) after treatment with HBO 2,4 ATA with 98% oxygen for three sessions with the duration of 30 min/session, and air brake for 5 min between each session for 10 days consecutively. Conclusion: The effect of eNOS on HIF-1α levels in HBOT shows that HBO can significantly affect the levels of HIF-1α through endothelial nitric oxide synthase (eNOS) level.

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