Rudi Yuwana, - (2001) IMMUNE RESPONSE MODULATION IN THE INTERNAL PUDENDAL ARTERY IN WI STAR RATS WITH STRESS CONDITION. Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

"It has long been stated that psychogenic stressors like anxiety, depression, and stress may cause Erecti le Dysfunction (ED), Many clinical studies have shown the relationship between psychogenic conditions and the development of ED (psychogenic ED). However, until recently. the exact mechanism of ED in patients with psychogenic strcsso rs had not Been understood. To clarify the pathogenesis of psychogenic ED, an animal experimental research using psychoneuroimmunology (PN!) paradigm was conducted. The design chosen was a Randomized Control Group PosHest only Design. Forty male rats, Rattus Nervigicus of Wistar strain were used and at random di vided into 4 groups often rats. Group I (control group for 5 days experim ent), group "" (5 days experiment group), group III (control group for 10 days experiment), group IV ( 10 days experiment group). The rats were 3-4 months old and had a body weight of 150-200 gr An electric footshock (EF) was used as a psychogenic stressor (as ind ependent variab le) exposed to the rats. The dependent variables observed were Immune Response Modulations (TRM) of the neurohormonal changes expressed in cort isol, cathecholamines (adrenal in, noradrenalin) blood level, amount ofVCAM-l expressing endothelial cells (ECs) and amount of IL-2, IFNy, IL-4, IL-IO producing lymphocytes in the intern al pudendal artery wall, the main artery for erection. A multivariate analysis was used to evaluate the data. The results after 5 days of EF exposure showed cortisol, adrenalin and noradrenalin blood levels increased; amount of VCAM- I expressing ECs increased and amount of lL-2, rFNg,. TL-4, I""L-IO producing lymphocytes decreased. After 10 days of EF exposure cortisol, adrenal in and noradrenalin blood levels also increased; amount ofVCAM-1 expressing ECs decreased and amount oflL- I, TFNg, IL-4, IL 10 producing lymphocytes were more decreased. To explain the mechanism, the contribution function of each variables to the development of ED is needed. Based on discriminant anal ysis, only 5 variables (cortisol, adrenal in, VCAM- I, IL-4, TL- I 0) have contribution in IRM pattern. It showed Ihat after 5 days of EF exposure, fRM caused an increased adrenal in function, but a decreased corti sol function. VCAM- I expressing ECs and lL-4, IL-IO producing lymphocytes functions were increased. After 10 days of EF exposure there was a prominent increased cortisol function, but a decreased adrenalin function. rL-4 and [L-IO producing lymphocytes functions were more increased whereas VCAM- I expressing ECs funct ion was decreased. In conclu sion: the increased adrenalin function after 5 days of EF exposure and increased VCAM-I expressi ng Ees function (according to the theoritical fTamework) may cause endothelial dysfu nct ion and potentially may cause endotheli al damage in the internal pudendal artery wall, and hence causes psychogenic ED. After 10 days of EF exposure cortisol function increased, which consequently caused increased IL-4 and IL- IO function. This condition, where Th2 cells are dominant may cause endothelial damage of the internal pudendal artery wall through ADCC (antibody-dependent cell-cytotoxicity) process, and hence may cause psychogenic ED."

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