Sinari Alfat Sunarko, - and Wiwied Ekasari, - and Suryani Dyah Astuti, - (2017) Antimicrobial effect of Pleomele angustifolia pheophytin A activation with diode laser to Streptococcus mutans. Journal of Physics: Conference Series, 853. pp. 1-6. ISSN 1742-6588

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Abstract

The main purpose of this research is to identify potential of Pheophytin A. as photosensitizer a agent to inactivate Streptococcus muttans using laser diode of 405nm. Pheophytina is known as chlorophyll derivate that losses magnesium ion at the center of porphyrin ring structure. In this research, phrophytin was extracted from Suji leaf (Pleomeleangustifolia). To determine the antimicrobial effect of treatments on S. mutans, samples were divided into three groups as follows: (1) Groups A(treated with Pheophytin A. and laser 405 nm at varying energy density of 2.5; 5, 7.5; 10.0; 12.5; 15.0; 17.5 and 20.0 J/cm2), (2) Group C-(negative control, no treated), (3) Group C+ (treated only with pheophytin). The experiments were repeated at least three times for each group. The results were analyzed using analysis of variance and the Tukey test. A P value ≤0.05 was considered to indicate a statistically significant difference. The decrement of percentage of number of bacterial colonyes growth was defined as: | (Σ sample colony - Σ control colony)/ Σ control colony | x 100%. The result showed that the incubation of Pheophytin A. using irradiation from laser diode of 405nm have a significant effect towards the decrement in bacterial growth. The most decreased percentage colony of S. mutans occurred on the incubation of pheophytin a treatment and laser irradiation 405nm with density 20 J/cm2 is 61.9%. This showed that pheophytin a functions as a photosesitizer activator to inactivate S. mutans bacteria.

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