Darmawati, - (2001) The Imun Respon Imunoglobulin M And Imunoglobulin G To Mice ( Mus Muscu/Us) After Immunization Prm Protein Dengue Virus. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Dengue virus (DEN) is an RNA of Flavivirus from Flaviviridae family, which is very pathogenic for human beings and spread rapidly through the bites of Aedes aegypti and Aedes alhopictus mosquitoes, particularly in tropical countries including Indonesia. More than 100 countries are seriously at risk for Dengue virus infection, and at least 20 million peoples a year are infected with the Dengue virus. The effort to control this disease has been done but not succeded. To date there is no tetravalent vaccine that can protect human from four virus strain that caused the disease. PrM protein is one of Dengue virus protein that can enhance to monocyte receptor which can stimulate B cell to produce antibody. This research intends to identify produce of eithe M and G antibody profile after immunization with PrM protein fraction of Dengue virus to mice. This research use 40 mice (Mus musculus) strain BALB/c which divided into three groups. Each group consist of 10 mice immunization with PBS as control, 15 mice immunization with PrM protein fraction dosage 500 gg each, and 15 mice immunization with PrM protein fraction dosage 1000 ps each. Mice serum was taking on 4, 7, 10, 22, and 30 days to analysis with ELISA to measure immunoglobulin concentration. The results showed that there was the real different about the M and G immunoglobulin concentration, and also between IgG, IgG 1, IgG2a, and IgG2b either 500 iv and 1000 .tg dosage.

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