Alexander Patera Nugraha, - and Nastiti Faradilla Ramadhani, - and Wibi Riawan, - and Igo Syaiful Ihsan, - and Diah Savitri Ernawati, - and Rini Devijanti Ridwan, - and Ida Bagus Narmada, - and Tania Saskianti, - and Fianza Rezkita, - and Andari Sarasati, - and Tengku Natasha Eleena Binti Tengku Ahmad Noor, - and Bilqis Inayatillah, - and Albertus Putera Nugraha, - and Florentina Joestandari, - (2022) Gingival Mesenchymal Stem Cell Metabolite Decreasing TRAP, NFATc1, and Sclerotin Expression in LPS-Associated Inflammatory Osteolysis In Vivo. European Journal of Dentistry. ISSN 1305-7456

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Abstract

Objective Bone is a dynamic tissue that undergoes remodeling. During bone remodeling, there are transcription factors such as nuclear factor-activated T cells-1 (NFATc1), sclerostin, and tartrate-resistant acid phosphatase (TRAP) that are released for bone resorption. Metabolite from gingival mesenchymal stem cells (GMSCs) has the ability to activate proliferation, migration, immunomodulation, and tissue regeneration of bone cells and tissues. Furthermore, the aim of this study is to investigate the metabolite of GMSCs’ effect on expression of NFATc1, TRAP, and sclerostin in calvaria bone resorption of Wistar rats. Materials and Methods Twenty male healthy Wistar rats (Rattus norvegicus), 1 to 2 months old, 250 to 300 g body were divided into four groups, namely group 1 (G1): 100 µg phosphate-buffered saline day 1 to 7; group 2 (G2): 100 μg lipopolysaccharide (LPS) day 1 to 7; group 3 (G3): 100 μg LPS þ 100 μg GMSCs metabolite day 1 to 7; and group 4 (G4): 100 μg GMSCs metabolite day 1 to 7. Escherichia coli LPS was used to induce inflammatory osteolysis on the calvaria with subcutaneous injection. GMSCs metabolite was collected after passage 4 to 5, then injected subcutaneously on the

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