Rahayu Kusdarwati, ,- and Muhamad Amin, ,- and Aldiansyah Budi Wardana, ,- (2021) DNase and Gelatinase Activities Of β -Hemolysin Aeromonas hydrophila Isolated from Catfish (Clarias batrachus ). Journal of Aquaculture and Fish Health, 10 (3). pp. 331-340. ISSN 2301-7309

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Abstract

Aeromonas hydrophila infection can cause mass mortality in fish and result in economic losses for farmers. These are bacterium that secrete substances or compounds that support its virulence by producing toxins and extracellular proteins in the form of enzymes. This study aimed to determine the activity of DNase enzyme, and gelatinase enzyme of β- hemolysin toxin A. hydrophila isolated from catfish (Clarias batrachus). This research was carried out in November 2020 in Dukuh Tengah Village, Buduran District, Wedoro Village, Waru District, Wadung Asri Village, Waru District and Keboan Sikep Village, Gedangan District. The sampling technique was carried out by researchers by following activities in the field and visiting correspondents directly in the catfish cultivator ponds, then the catfish samples were carried out by biochemical tests and toxin and enzyme activity tests to obtain the information and data needed. The results showed that A. hydrophila bacteria in catfish produced positive β-hemolysin toxin, DNase enzyme, and gelatinase enzyme. The research concluded that 10 isolates of A. hydrophila bacteria isolated from catfish (C. batrachus) in Sidoarjo showed the activity of β hemolysin toxin, DNase enzyme, and gelatinase enzyme

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