AYU PUSPITARANI, ,- and Woro Hastuti Satyantini, ,- and Rahayu Kusdarwati, ,- (2016) Pengaruh Pemberian Ekstrak Air Panas Spirulina platensis Pada Pakan Terhadap Kelangsungan Hidup dan Total Hemosit Udang Vaname Setelah Diinfeksi dengan Vibrio harveyi. Journal of Aquaculture and Fish Health, 5 (3). pp. 92-99. ISSN 2301-7309

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	Text (Ethical Clearance) Ethical 25. Pengaruh Pemberian Ekstrak Air Panas Spirulina platensis pada Pakan Terhadap Kelangsungan Hidup dan Total Hemosit Udang Vaname Setelah Diinfeksi dengan Vibrio harveyi..pdf Download (6MB)
	Text (Artikel) Artikel 25. PENGARUH PEMBERIAN EKSTRAK AIR PANAS Spirulina platensis PADA PAKAN TERHADAP KELANGSUNGAN HIDUP DAN TOTAL HEMOSIT UDANG VANAME SETELAH DIINFEKSI DENGAN Vibrio harveyi.pdf Download (579kB)
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Abstract

White leg shrimp (Litopenaeus vannamei) is the one commodity that has an important economic value. Production of white leg shrimp (Litopenaeus vannamei) had many problems due to disease outbreaks. One is causes from Vibrio harveyi bacteria. This bacteria causes high mortality. To overcome this problem, commonly used antibiotics but the impact of is on the emergence of bacterial resistance and residues in shrimp. Applications use of natural materials as immunostimulant Spirulina platensis in the form of hot water extracts known to have a stimulatory effect to enhance the immune response in white leg shrimp (Litopenaeus vannamei). This immune response is shown by the increasing number of total haemocytes count that directly affect the value of survival rate. This study aimed to determine the effect of hot water extract of Spirulina platensis on the feed to the total haemocytes (Total Haemocyte Count) and survival ratewhite leg shrimp after challenged tested with Vibrio harveyibacteria. The method used is an experimental method completely randomized design (CRD) with 5 treatments (0 mg/kg, 200 mg/kg, 400 mg/kg, 600 mg/kg, and 800 mg/kg) and 4 replications. White leg shrimp(Litopenaeus vannamei) given feed containing hot water extract of Spirulina platensis for 14 days later on the 15th day of shrimp challenged tested with Vibrio harveyi were injected with a dose of 106 cell/ml in the abdominal segment. Observation of the total number of hemocytes (Total Haemocyte Count) is on days 0, 14th, 16th and 26th. Observation of the value of survival rate was did for ten days after the shrimp infected. The results showed an increase in the total number of haemocytes in white leg shrimp (Litopenaeus vannamei) due to the provision of hot water extract of Spirulina platensis compared with control (without hot water extract of Spirulina platensis). Hot water extract of Spirulina platensis also effectively improve the survival rate white leg shrimp post-infection with Vibrio harveyi bacteria with the best treatment at a dose of 800 mg / kg diet.

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