Mat Taib Mimi Iryani, ,- and Patrick Sorgeloos, ,- and Muhd Danish-Daniel, ,- and Min Pau Tan, ,- and Li Lian Wong, ,- and Wen Jye Mok, ,- and Woro Hastuti Satyantini, ,- and Gunanti Mahasri, ,- and Yeong Yik Sung, ,- (2020) Cyst viability and stress tolerance upon heat shock protein 70 knockdown in the brine shrimp Artemia franciscana. Cell Stress and Chaperones, 25 (1). 1099 -1103. ISSN -

	Text (Peer Review) C7.pdf Download (1MB)
	Text (Artikel) Artikel 8. Cyst viability and stress tolerance upon heat shock protein 70 knockdown in the brine shrimp Artemia franciscana.pdf Download (1MB)
	Text (Plagiasi) TURNITIN_C8_Cyst Viability.pdf Download (1MB)
	Text (Penilaian Kualitas Karil dan Kesesuaian Bidang Ilmu) KARIL_C8_Cyst Viability.pdf Download (122kB)
	Text (Korespondensi) KORESPONDENSI_C8_Cyst viability and stress tolerance upon heat shock protein 70 knockdown in the brine shrimp Artemia fransiscana.pdf Download (474kB)
	Text (Ethical Clearance) Ethical 8. Cyst Viability and Stress Tolerance Upon Heat Shock Protein 70 Knockdown in the Brine.pdf Download (7MB)

Abstract

Females of the brine shrimp Artemia franciscana produce either free-swimming nauplii via ovoviviparous pathway of reproductionorencystedembryos,knownascysts,viaoviparouspathway,inwhichbiologicalprocessesarearrested.Whileprevious study has shown a crucial role of ATP-dependent molecular chaperone, heat shock protein 70 (Hsp70) in protecting A. franciscana nauplii against various abiotic and abiotic stressors, the function of this protein in diapausing embryos and cyst development, however, remains unknown. RNA interference (RNAi) was applied in this study to examine the role of Hsp70 in cystdevelopmentandstresstolerance,withthelatterperformedbydesiccationandfreezing,acommonmethodusedfordiapause termination in Artemia cysts. Hsp70 knockdown was apparent in cysts released from females that were injected with Hsp70 dsRNA.ThelossofHsp70affectedneitherthedevelopmentnormorphologyofthecysts.Thetimebetweenfertilizationandcyst release from Artemia females injected with Hsp70 dsRNAwas delayed slightly, but the differences were not significant when comparedtothecontrols.However,thehatchingpercentageofcystswhichlacksHsp70werereducedfollowingdesiccationand freezing.Takentogether,theseresultsindicatedthatHsp70possiblyplaysaroleinthestresstolerancebutnotinthedevelopment of diapause-destined embryos of Artemia. This research makes fundamental contributions to our understanding of the role molecular chaperone Hsp70 plays in Artemia, an excellent model organism for diapause studies of the crustaceans.

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