Akhmad Taufiq Mukti, ,- and SEPTURESTY HARTRI EKA, ,- and Woro Hastuti Satyantini, ,- and Ahmad Shofy Mubarak, ,- (2020) Studi Pendahuluan: Pengaruh Krioprotektan yang Berbeda terhadap Persentase Kerusakan dan Penetasan Embrio Ikan Lele Dumbo (Clarias gariepinus). Journal of Aquaculture and Fish Health, 9 (3). pp. 214-221. ISSN 2301- 7309

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	Text (Ethical Clearance) 14. Studi Pendahuluan Pengaruh Krioprotektan yang Berbeda terhadap Persentase Kerusakan dan Penetasan Embrio Ikan Lele Dumbo (Clarias gariepinus).pdf Download (9MB)
	Text (Korespondensi) C14_KORESPONDENSI_Preleminary Study The Effects of Different Cryoprotectants on Damage and Hatching Rates of African Catfish (Clarias gariepinus).pdf Download (501kB)
	Text (Artikel) 13. Studi Pendahuluan Pengaruh Krioprotektan yang Berbeda terhadap Persentase Kerusakan dan Penetasan Embrio Ikan Lele Dumbo (Clarias gariepinus) (1).pdf Download (1MB)
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Abstract

This study was aimed to observe the effects of different cryoprotectants in embryo cryopreservation on damage and hatching rates of African catfish (Clarias gariepinus) embryo. The gastrula-staged embryos were treated 5% (v/v) solutions concentration of dimethyl sulfoxide (DMSO), propylene glycol (PG), honey, and combined cryoprotectants, respectively and preserved at temperatures of 4 and 0ºC for 30 min, 1 h, 2 h, 3 h, 4 h, 5 h, and 6 h, respectively. Thawing of embryos was conducted in freshwater at a temperature of 28ºC. Then, the embryos were incubated in the aquaria at 28ºC temperature. The result showed that the different cryoprotectants in gastrula-staged embryo cryopreservation affect damage and hatching rates of African catfish embryos at different length of preservation. The percentage of catfish embryo damage increases with different length of preservation and the storage temperature. A combination of DMSO-honey and PG-honey has the lowest damage percentage and the highest hatching rate of catfish embryo compared to other treatments (p<0.05).

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