Ahmad Yudianto, - and Arofi Kurniawan, - and Fery Setiawan, - and Abdul Hadi Furqoni, - and Beta Novia Rizky, - (2022) Paternity Test Through Kinship Analysis and Cell Free Fetal DNA (Cff-DNA) as a Forensic Identification Technique. Journal of International Dental and Medical Research, 15 (4). pp. 1434-1442. ISSN 1309-100X

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Abstract

The application of kinship analysis and cell-free fetal DNA (cff-DNA) analysis can be found in forensic identification processes, mainly on (civil or criminal) parentage testing, disaster victim identification, missing person identification, and familial searching (Butler, 2006; 2015). So far, paternity tests using kinship analysis and cell-free fetal DNA (cff-DNA) as a forensic identification examination are still not widely known. This study aims to analyze the application of kinship analysis and cff-DNA analysis in paternity examination tests. This study is observational laboratory research with a one-shot research design. The extracted DNA sample was measured for its DNA contents and purity. The average DNA contents were 575 ± 4.33 ng/μl with the purity range of 1.035-1.82 while the average DNA contents for maternal plasma DNA (cff-DNA) were 75 ± 2.31 ng/μl with the purity range of 1.12-1.56. The highest allele frequency was on allele 31 of the D21S11 locus (64.375%). All the examined STR CODIS loci showed that allele sharing was dominated by two allele sharing with a percentage higher than 50%. The 13 STR CODIS loci has the highest percentage of two allele sharing. Based on this finding, it is recommended that paternity tests can be performed through kinship line by using siblings’ DNA in case the DNA from the parents are unavailable, and the use of cff-DNA as a non-invasive method in paternity test examination.

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