Ni’matuzahroh, .- and Rima Nurmalasari, .- and Rozy Ayu Silvia,, .- and Tri Nurhariyati,, .- and Tini Surtiningsih, .- (2015) Effectiveness in Enhancing Oil Recovery through Combination of Biosurfactant and Lipases Bacteria. Journal of Applied Environmental and Biological Science, 5 (6). pp. 83-87. ISSN 2090-4274

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Abstract

Oil pollution accidents recently became phenomenon and caused accidental contamination of ecosystem. It originated from leaking pipes, transportation accidents, and damage oil storage tanks that contaminated both soil and groundwater. One of the solution was oil recovery process using sand pack column with combination of biosurfactant (Bacillus subtilis 3Kp, Pseudomonas putida T1-8, Micrococcus sp. L II 61 and Acinetobacter sp. P2(1)) and lipases (Actinobacillus sp., Micrococcus sp. L II). Sand-pack column model designed to simulate oil recovery operations and evaluate the mobilization of residual oil by combined biosurfactants and lipases. This study was an experimental study through four replications. Treatment were consists of eight combinations; Acinetobacter sp. P2 (1) - Actinobacillus sp., Bacillus subtilis 3Kp - Actinobacillus sp., Pseudomonas putida T1-8 - Actinobacillus sp., Micrococcus sp. L II 61 - Actinobacillus sp., Acinetobacter sp. P2 (1) - Micrococcus sp. L II 61, Bacillus subtilis 3Kp - Micrococcus sp. L II 61, Pseudomonas putida T1-8 - Micrococcus sp. L II 61 and biosurfactant and lipase Micrococcus sp. L II 61 with positive control synthetic surfactant (Tween-20). The results of treatment were extracted using n-hexane. Effectiveness of oil recovery by biosurfactants and lipases combination analyzed using One-way ANOVA test. These eight combinations effectively mobilize of entrapped oil as indicated by high percentage of oil recovered compared to the synthetic surfactants (Tween-20). Thus, the eight types of combination were capable to replace synthetic surfactants in oil recovery processes using sand pack column.

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