Syahriar Nur Maulana Malik Ibrahim, .- and Sibyandhita Erhaa Amalia, .- and Bunga Agatha, .- and Mustikayanti Yuni Lestari, .- and Antonius Dwi Saputra,, .- and Nur Laily Fachira Ikmala, .- and Fazareyna Hyuwan Argi Ryanto, .- and Puspita Ayu Dewi, .- and Mochamad Khoirul Anam, .- and Farah Aisyah Nafidiastri, .- and Nastiti Trikurniadewi, .- and Ditta Putri Kumalasari, .- and Erta Tri, .- and Gendut Hariyanto, .- and Suwarni, .- and Agus Supriyanto, .- and Ni’matuzahroh (2017) Screening and identifying of cellulolytic bacteria from Alas Purwo National Park. In: Inventing prosperous Future Through Biological Research and Tropical Biodiversity Management: Proceedings of the 5th International Conference on Biological Science,. Proceedings of the 5th International Conference on Biological Science, 2002 . AIP Publishing., Yogyakarta Indonesia, pp. 1-7. ISBN 978-0-7354-1718-2

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Abstract

This research aimed to screen and identifies cellulolytic bacteria from Alas Purwo National Park. Soil samples were taken from five points in each location by indirect sampling method. Bacteria were isolated with CMC (Carboxymethylcellulose) agar. The result had successfully obtained 16 isolates which produced cellulase. The cellulase activity index assay of bacteria used CMC agar modified with adds 1 % YME (Yeast Malt Extract) and then was incubated during one day at room temperature. Bacterial colonies were soaked with Congo red 1 % and rinsed with NaOH 10 % to visualize the halo zone formed. Three isolates with highest cellulase activity index were identified by observing the colony morphology, cell morphology, and the biochemical characters. The results of this research showed that the three highest potential isolates producing cellulase were BPA-B, BSA-D, and BPA-A with cellulase activity indexes respectively 10.67, 9.86, and 8.16. BPA-B, BSA-D, and BPA-A each have the similarity with genera Pimelobacter, Micrococcus, and Cellulomonas.

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