Martono Tri Utomo, Martono and Subijanto M. Sudarmo, - and Ketut Sudiana, Ketut Zinc Supplementation Effect in MDA and MMP8 During LPS Induced Sepsis and Its Benefits to Reduce The Child Mortality in Hospital Practice. International Journal of Research Publication, 85 (1). pp. 23-30. ISSN 2708-3578

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Abstract

Abstract Background: Severe sepsis increases pro-inflammatory cytokines, causing systemic translocation to the commensal bacteria. A study in Southeast Asia involving 13 public hospitals in 3 countries, including Indonesia, found that sepsis mortality in children was 2% and a 28% incidence of severe sepsis. Low plasma zinc levels were found in severe and critically septic patients in children and adults. Objective: This study aims to explain the mechanism of sepsis improvement after zinc administration through MDA and MMP8 during LPS-induced sepsis in rodents. Material and Methods: We divided samples of 40 rats into four groups of Control, LPS, LPS-Zinc, and Zinc. Blood sampling in 2-hour after LPS or placebo administration to measure zinc level. Zinc was administered in LPS-Zinc and Zinc group and placebo in two other groups. Blood sampling in 8, 24, and 72 hours to measure MDA and MMP-8 by sandwich-ELISA method. Statistical analysis was carried out with one-way ANOVA, Kruskal Wallis, Mann-Whitney, Paired t-tests, Wilcoxon Signed Rank Test, and path analysis. Results: There were decreased in MDA, and MMP-8 serum levels in the LPS-Zinc group compared to the LPS group. Conclusion: Administration of zinc in sepsis improves the condition of sepsis by decreasing pro-inflammatory cytokine and increasing anti-inflammatory cytokine

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