Ria Pingkan Ganda, - and Aryati, - and Sidarti Soehita, - (2021) Suitability Tests for Bacterial Identification and Antibiotic Sensitivity Tests using Microscan Walkaway on Vitek 2. Indian Journal of Forensic Medicine & Toxicology, 15 (2). pp. 3985-3991. ISSN 9739122

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Abstract

Culture or bacterial culture is the gold standard for diagnostic examination to detect the presence of microorganisms in the patient’s body. The development of culture technology and culture-based automatic devices for diagnostic examination are widely researched. At present, there are several types of automatic blood culture instruments in Indonesia, namely Vitek 2 and Microscan Walkaway. This research aims to analyze the suitability of bacterial identification and test antibiotic sensitivity of Microscan Walkaway device from Beckman Coulter and Vitek 2 device from Biomerieux as a gold standard. The research conducted was an observational cross-sectional study. Sampling was done consecutively. The study sample consisted of 202 samples obtained from the results of positive isolates, during January-July 2019 at Dr. Soetomo hospital. Positive isolates were examined for bacterial identification and antibiotic sensitivity using Vitek 2 and Microscan Walkaway. The results were statistically analyzed using SPSS. Examination of bacterial identification using the Microscan device showed 34.2% of Gram-positive bacteria and 65.8% of Gramnegative bacteria, whereas with Vitek 2, results showed 34.7% of Gram-positive bacteria and 65.3% of Gram-negative bacteria. Both of these instruments showed identification accuracy of 98.56% for Gram positive bacteria and 100% for Gram negative bacteria with Kappa value: 0.814 and p <0.0001. The results of the accuracy test for antibiotic sensitivity of multidrug resistance bacteria showed compatibility with p value <0,0001. There is very good agreement between Vitek 2 and Microscan Walkaway in the identification of bacteria and antibiotic sensitivity.

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