Firdausy Kurnia Maulana, - and Didik Handijatno, - and Hani Plumeriastuti, - (2019) Detection of 37 kDa Outer Membrane Protein H (ompH) gene of Pasteurella multocida Type B Local Isolate From Nusa Temggara Timur (NTT). Biomedical, Reproduction, Nutrition and Clinical Science, 10 (12). pp. 1708-1713. ISSN 0976-0245

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Abstract

Haemorrhagic Septicaemia (HS), in Indonesia known as Ngorok disease is a fatal acute septicemia disease in cattle and buffalo caused by Pasteurella multocida. Omp has a number of important roles for bacterial cells, such as uptake of nutrients, transporter for molecules in and out of cells and interactions with the host and environment. Outer membrane Protein H (OmpH) in P. multocida is one of the major proteins in the envelope which has been purified and characterized as porin immunodominant. The purpose in of this study was to detect the 37d kDa outer membrane protein H (ompH) gene from P. multocida local isolate from Nusa Tenggara Timur (NTT) and compare it with vaccine strain. Detection of 37 kDa ompH of P. multocida was done using PCR. Sample used in this study were P. multocida local isolate from NTT and vaccine strain (Katha). Isolation and identification were performed using bacteriological culture and biochemically characterization. Results from PCR showed a 37 kDa ompH gene on P. multocida local isolate and vaccine strain is 946 bp

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