Budiastuti, - and Niken Dwi Lestari, - and Mustofa Helmi Effendi, - and Arimbi, - and Hani Plumeriastuti, - (2020) Cytotoxic Effect of Essential Oil from Cinnamon (Cinnamomum burmannii) Bark on Rat Bone Marrow Mesenchymal Stem Cells: In Vitro Study. Journal Systematic Reviews in Pharmacy, 11 (9). pp. 378-383. ISSN P-ISSN 0975-8453, E-ISSN 0976-2779

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Abstract

The aim of this study was to determine the cytotoxicity properties of essentialoil from Cinnamon (Cinnamomum burmannii) bark on rat bone marrow mesenchymal stem cells (rBMSCs). This research was an experimental laboratory with The Post Test Only Group as a research design. Essential oil from Cinnamon (Cinnamomum burmannii) bark obtained from steam distillation method and essential oil was made in a series dilution with a concentration of 0,5%, 0,25%, 0,125%, 0,0625% and 0,0312%. Furthermore, the cytotoxicity test on rBMSCs using MTT assay. Microplate containing rBMSCs that had been exposed to five concentrations was incubated in a 5% CO2 incubator with 37 °C for 24 hours. The result of MTT assay can be seen from the absorbent solution formazan crystal through ELISA reader with the specific wavelength of 595 nm which produces data in the form of Optical Density (OD). The result research showed that the rBMSCs cell life percentage in 0,5%, 0,25%, 0,125%, 0,0625% and 0,0312% of concentration respectively were 12,8%, 18,3%, 21,7%, 26,5% and 32,5%. Probit analysis is used to determine the LC50 of essential oils from Cinnamon (Cinnamomum burmannii) bark. It can be concluded that the smaller the concentration of essential oils given to rBMSCs, the higher the percentage of cell life. In this study, the concentration of essential oils which can kill 50% of rBMSCs was 0,004%

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