Isnin Anang Marhana, - and Muhammad Amin, - and Gondo Mastutik, Gondo and Mokhammad Mukhlis, - Expression of MAGE-A3 in and histopathological analysis of forceps biopsy specimens of non– small-cell lung carcinoma patients. Pneumologia, 71 (1). pp. 14-21. ISSN pISSN:2067-2993, eISSN 2247-059X

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Abstract

English:Non–small-cell lung cancer (NSCLC) is a type of epithelial lung cancer and associated with cigarette smoking (passive or active).Melanoma-associated antigen 3 (MAGE-A3) is widely expressed in various types of tumours, including NSCLC. This studyaimed to examine the MAGE-A3 expression in forceps biopsy specimens as a tumour biomarker to be used for early diagnosisand screening of lung cancer. This study was an observational, analytical study with a cross-sectional study design. The samplesize was determined based on Ronald Fisher’s classic z transformation formula, and samples were selected using consecutivesampling. The study population included 14 lung tumour patients. Samples were obtained by forceps biopsy with bronchoscopyguidance. Histopathological analysis was carried out using Giemsa staining. The expression of MAGE-A3 was determined usingRT-PCR. All data were analysed using SPSS statistics software (IBM SPSS Statistics, IBM® SPSS® Statistics is a powerfulstatistical software platform RRID: SCR_019096). In this study, there were 6 subjects (42.9%) with NSCLC adenocarcinomaand 8 subjects (57.1%) with squamous cell carcinoma. The positive MAGE-A3 expression was found in 5 (35.7%) of the totalresearch subjects, and the expression on RT-PCR analysis was at 569 bp. We found that MAGE 3 gene was mostly expressed inadenocarcinoma of NSCLC, even though there was no statistical correlation with histopathological results (P > 0.05). MAGE-A3expression in forceps biopsy specimens of NSCLC was mostly found in the adenocarcinoma type at 569 bp. Therefore, it couldbe used as a tumour biomarker for early diagnosis and screening of lung cancer.

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