Suryo Kuncorojakti (2010) Efektifitas Antibodi Anti-Hemaglutinin Asal Kuning Telur Igy) sebagai Agen Imunoterapi pada Ayam yang Terinfeksi Virus Avian Influenza A/H5Nl. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

This study was aimed to know; (1) the reactivity anti-HA antibody Avian influenza virus subtype H5Nl, (2) the blockage mechanism of antibody anti-HA in the cell tropism by blocking specific reseptor SA alfa 2,3 gal, (3) the antibody anti-idiotype formation in the chickens after therapy using anti-HA antibody. This study was devided into three step; The first step were hemaglutinin protein caracterisation by SDS-P AGE and western blot, electroelution of hemaglutinin protein, quantitation, immunogenicity and antigenicity test. The second step were anti-HA antibody isolation from the egg yolk, antibody purification and reactivity test by dot blot method. And the third step were challenge test and artificial infection, anti-idiotype antibody detection (anti anti-HA), immunohistochemistry test for detecting Avian influenza virus antigen and anti-HA antibody blocking in the cell tropism. The result of this study showed that; (1) Anti-HA antibody can be detected Avian influenza AlH5Nl virus antigen and protect the chicken from Avian influenza AlH5Nl virus with the protectivity level up to 100%, (2) The protectivity mechanism to the infection was by blocking the receptor SA alfa 2,3 gal, (3) anti-idiotype antibody was not detected after using anti-HA antibody as an immunotherapy agent in the chicken.

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