Reza Mahendra Yudha Permana, - (2015) Pengaruh Suplementasi Insulin Transferrin Selenium (Its) Pada Media Maturasi Tcm-L99 Terhadap Diameter Dan Ekspresi Maturation Promoting Factor (Mpf) Oosit Sapi. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Insulin Transferrin Selenium (ITS) has been used in vitro maturation system to support in vitro maturation of oocyte. The study aims was to detennine role ofInsulin Transferrin Selenium to bovine oocyte was matured in vitro against improved oocyte diameter and Maturation Promoting Factor (MPF) as maturation mediator by immunocytochemistry using strepravidin biotin complex method. Maturation process used TCM-199 medium which added by FSH 100 IU/ml, LH 100 IU/ml, BSA 3% and ITS (Insulin Transferrin Selenium). ITS dose which used in culture medium are different for each treated group. The treated groups are Po (0 ml) PI (10 ml) P2 (15 ml), and p, (20 ml) Oocytes were incubated in incubator at 38,5°C with C02 5% and humidity 95% due 22 hours. The result showed that ITS supplementation in maturation medium has no significantly difference (p>0,05) for all treated groups (Po, PI, P2, and P,) of oocyte diamter improvement. In contrary, ITS supplementation Po (0 ml) has significantly difference (p<0,05) compared PI (10 ml), P2 (15 ml) and P3 (20 ml) of MPF expression but MPF expression of PI (10 ml), and P2 (15 ml) has no significanly difference (p>0,05). In conclusion of ITS supplementation in maturation medium TCM-I99 can't increase oocyte diameter but can increase MPF expression of bovine oocyte were matured in vitro.

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