Anwar Ma'ruf, - and Romziah Sidik, - and Ratna Damayanti, - (2010) Potensi Protein STAT (Signal Transducers and Activators of Transcription) Sebagai Kandidat Bahan Pemacu Pertumbuhan Ternak. Other thesis, UNIVERSITAS AIRLANGGA.

Text Dr ANWAR MA RUF M Kes drh.pdf Download (4MB)

Abstract

The Long-Term Objective Of This Study Was To Produce STAT Synthetic Protein In Broiler During Growth Period Resulting From The Increase Of Growth Hormone (GH) As Growth Promoter. This Study Used Ten Male Broiler Lohrilan (MB 202 P) From PT. Multibreeder Indonesia Tbk. Broilers Were Kept Within Batteried Cage, With A Capacity Of One Broiler In Each Cage. The Broilers Were Fed Twice A Day, At 6 A.M. And 6 P.M. With The Amount Of Feed 10% Less Than Standard. At Day 21 The Broilers Were Sacrificed To Obtain The Samples, I.E., Adipose Tissue, Liver And Muscles (M. Femoralis And M. Pectoralis) For The Following Examinations: (1) Isolation Of STAT-3 Signaling Protein From Adipose Tissue, Liver, And Muscles (M. Femoralis And M. Pectoralis) Of The Broiler, (2) Analysis Of STAT-3 Signaling Protein From Adipose Tissue, Liver, And Muscles (M. Femoralis And M. Pectoralis) Of Tr.E Broiler Using SOS-PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoreses) Method, And (3) Identification Of STAT-3 Signaling Protein Using Western Blot Method By Means Of Protein Detection Using Electrophoresis With Potyacrylamide Gels. Results Of Examination On Protein In Hepatic Tissue, Muscle And Adipose Tissue Of..~Roilers In Growth Period Using Dot Blot Revealed That STAT Protein Was Positively Present In Those Tissues. This Finding Was Followed-Up With SOS-PAGE Examination, From Which We Found The Presence Of Protein Band Between The Markers Of 116 Koa And 14.4 Koa. The Protein Band Was Supposedly The STAT-3 Protein. To Prove That Protein Band Formed Was The STAT-3, Western Blot Examination Was Conducted Using Rabbit Polyclonai Antibody STAT-3. The Result Showed The Formation Of The Protein Band, Indicating The Presence Of Reaction Between Antigen (STAT-3 Protein) And STAT-3 Protein Antibody. In Conclusion, STAT -3 Protein Is Present In Hepatic, Muscular, And Adipose Tissues, With Molecular Weight Of 59.4 Koa.

Actions (login required)

View Item