Muhammad Khaliim Jati Kusala (2024) Cross Reactivity Between H5 Clade 2.1.3 Serum And H5 Clade 2.3.2 Virus By Using Serum Netralization Test. Skripsi thesis, UNIVERSITAS AIRLANGGA.

Text (fulltext) CROSS REACTIVITY BETWEEN H5 CLADE 2.1.3 SERUM AND H5.pdf Download (4MB)

Abstract

The aim of this research was to know the cross-reactions between avian influenza virus clade 2.1.3 and 2.3.2 to 2.1.3 clade avian influenza serum by using serum neutralization test also to know the effectivity avian influenza clade 2.1.3 vaccine against 2.1.3 and 2.3.2 virus in vitro. This research was held at Avian Influenza Research Center (AIRC) with the laboratory Biosafety Level-2 (BSL-2) Universitas Airlangga. This research was held from June to July 2014. Serum of H5Nl clade 2.1.3 that be used had the titre of 27 by Haemaglutination Inhibition (HI) assay. The method of this research is using H5 clade 2.1.3 serum stock AIRC Universitas Airlangga that be tested against H5 clade 2.1.3 (A/CklInd/114/08) and 2.3.2 (AlDklInd/443/12) virus by using Serum Neutralization Test assay. The TCID50 of the viruses are 1.78 x 108 TCID50/ml of H5 clade 2.1.3 and 5.62 x 107 TCID50/mi of H5 clade 2.3.2. Serum was tested with both viruses by using serum neutralization test (SNn assay in the MOCK cell culture. The data was analyzed by Reed and Muench Formula. The result of SNT showed that the serum were able to neutralize and protect at least 50% of cell culture at the 1: 160 dilution to H5 clade 2.1.3 virus. While serum tested with H5 clade 2.3.2 virus on SNT, it was showed that there were cross reaction, the serum was able to neutralize the virus and protect at least 50% of cell culture at the 1:1258 dilutions to H5 clade 2.3.2 virus.

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