Ni Nyoman Tri Puspaningsih, - and Eddy Bagus W., - and A.A. Istri Ratnadewi, - and Anita Kurniati, - and Laura Navika Yamani, - (2011) Peningkatan Aktivitas Enzim Hemiselulase Dengan Rekayasa Protein Untuk Pengolahan Biomassa Berbasis Lignoselulosa. PASCASARJANA, SURABAYA. (Unpublished)

Text (FULLTEXT) 85. PENIGNKATAN AKTIVITAS ENZIM HEMISELULASE DENGAN REKAYASA PROTEIN.pdf Download (69MB)

Abstract

The purpose of this research were to investigate surface structure modification and crystallinity of rice straw and water hyacinth after recombinant o-Larabinofuranosidase treatment. Analysis of surface structure modification and crystallinity were done by SEM and XRD. In the process of rice straw and water hyacinth hydrolysis were determined optimation of intracellular enzyme (P) and extracellular enzyme mixture ratio and optimation of incubation time. The optimum ratio of rice straw was 1:1 and water hyacinth was 1:2. The optimum incubation time of rice straw and water hyacinth hydrolysis was 8 hours. Rice straw and water hyacinth after recombinant -1 -arabinofuranosidase treatment were analyzed by SEM and XRD, Rice straw and water hyacinth before recombinant a-L-arabinofuranosidase treatment were used as control. The conclusion of this research indicated that enzymatic hydrolysis damaged surface structure of rice straw and water hyacinth. Enzymatic hydrolysis showed effect on cristallinity of water hyacinth.

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