Yenny Candra Christinasari, - (2011) Isolasi Dan Identifikasi Serta Uji Kepekaan Bacillus Subtilis Dar I Sedimen Tambak Udang Terhadap Bahan Antimikrobial. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

The aims of this study was to know the characteristic and the sensitivity of Bacillus subtilis isolated from shrimp culture ponds to antimicrobial agents. Fifty isolates of Bacillus spp. that fetmented mannitol on MSA media and showed rod shapes, had been identified by macroscopic, microscopic, biochemical and sugars test. The result showed six isolates were Bacillus subtilis. Antimicrobial agents that had been used for sensitivity test were Penicillin, Ampicillin, Oxytetracycline, Gentamicin, Ciprofloxacin, and Sulfamethoxazole by in vitro using Kirby-Bauer method. Disc of these antimicrobial agents were placed on the cultures of Bacillus subtilis at the surface ofMHA media, incubated at 3TC for 24 hours. Diameter of inhibitor area around the disc was measured on mm to identiJY the isolates resistant or sensitive. The sensitivity test result that Bacillus subtilis sensitive to alI of the antimicrobial agents with range of diameter inhibition was 16 until 38 mm. It means that Bacillus subtilis didn't have gen resistant and couldn't transfer it to pathogen bacteria in shrimp culture so that it was very safe for the use as a probiotic. It was suggested to do another identification test and do in vivo study to know the isolates safe to be used as a probiotic or not.

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