Yuyun Pusposari, Y (2004) Validasi Metode dan Penetapan Kadar Kuersetin dalam Ekstrak Etanol Daun Jambu Biji (Psidium guajava I.) Secara KLT-Densitometri. Skripsi thesis, UNIVERSITAS AIRLANGGA.
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Abstract
Quercetin, with various biological activities, are considered as a key compounds in Psidium guajava. A simple and reliable TLC-densitometry method had been developed for determination of quercetin in ethanol extracts of psidii folium. TLC on silica gel plates with chloroform-acetone-formic acid (150: 33: 17) v/v as mobile phase. Qualitative analysis was performed by spraying with AlCl3 5% solution in methanol and visualization under UV 365 nm. The mobile phase enables good resolution with Rs values were 5,4 and 4,9. Quantification by densitometry at 375 nm. Regression coefficient (r=0,9990), precission (CV<3%), recovery (96,70%-110,05%), detection limit (0,21 μg), and quantitation limit (0,64 μg) were validated and found to be satisfactory. This method was applied to 96%, 70% and 50% ethanol extracts of psidii folium and showed quercetin content were (2,94 ± 0,07)%, (2,96 ± 0,06)%, and (2,48 ± 0,04)%, respectively.
| Item Type: | Thesis (Skripsi) | |||||||||
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| Uncontrolled Keywords: | quercetin, TLC-densitometry, psidii folium extract, quantitative, qualitative | |||||||||
| Subjects: | R Medicine > RS Pharmacy and materia medica > RS1-441 Pharmacy and materia medica | |||||||||
| Divisions: | 05. Fakultas Farmasi > S1 Farmasi | |||||||||
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| Depositing User: | indah rachma cahyani | |||||||||
| Date Deposited: | 21 Feb 2025 08:40 | |||||||||
| Last Modified: | 21 Feb 2025 13:13 | |||||||||
| URI: | http://repository.unair.ac.id/id/eprint/136467 | |||||||||
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