Nailul Izzah, ,- (2013) Potensi Imunostimulan Ekstrak Tanaman Sambiloto (Andrognzphu paniculata) Terhadap Ekspresi IFN-y Dan TNJ'-a Pada PBMC (Peripheral Blood Mononuclear Cell) Ikan Bawal (Colossoma macropomumt Secara In Vitro : Penelitian Eksperimental Laboratoris. Thesis thesis, UNIVERSITAS AIRLANGGA.

Text (Fulltext) 178. POTENSI IMUNOSTIMULAN EKSTRAK TANAMAN SAMBILOTO - NAILUL IZZAH - TIDAK ADA.pdf Download (2MB)

Abstract

This study was aimed to detennine the potential dose of sambiloto extract (Andrographis paniculata) as an immunostimulant in PBMC cachama (Colossoma macropomum) on the expression of IFN-y and TNF-a by in vitro. Blood from a 8 months cachama with 600 grams weight was used for samples. 10 ml of a cachama blood was taken and grouped into 30 well to incubated for two days with doses 5 mg I ml, I 0 ug I ml, 15 ug I ml, 20 ug I ml, 25 ug I ml sambiloto extracts and a control treatment which was given the sambiloto extract solvent (DMSO) without any sambiloto extract as a comparison. Results in PBMC cultures showed that sambiloto extracts could induce the expression of various cytokines and cells because it had an active ingredient diterpenoid and flavonoids which was indicated by a cell growth in culture media. In the chemical immunocytochemistry test, sambiloto extract (Andrographis paniculata) proved the capability of being immunostimulatory cachama PBMC which could express IFN-y and TNF-a (Colossoma macropomum). The average percentage of IFN-y and TNF-a were expression in PBMC cells for Pl group (dose 5 mg I ml) showed the highest results are 97.9% and it was 96.4% significantly different (p <0.05) when compared with the treatment group PO (control treatment), P3 (dose of 15 mg I ml), P4 (dose of 20 mg I ml) and P5 (dose of 25 mg I ml), but were not significant difference (p> 0.05) when compared with P2 (dose of 25 ug I ml). A.paniculata extracts could produce IFN-y from NK cells, CD8 cells, CD4 Thi cells and also activate macrophages to release IL-12 and TNF-a. As a conclusion from this study can be avowed that the sambiloto extract (Andrographis paniculata) can take the role as an immunostimulant in PBMC cachama ( Colossoma macropomum) which is indicated by the presence of IFN-y expression lymphocytes and TNF-u expression monocytes by in vitro at the optimum dose 5 mg I ml. In addition, it needs a further research by in vivo and a test with a variety of diseases.

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