Sutanto, Laurencia Gabrielle and Jiwanti, Prastika Krisma and Saputra, Mirza Ardella and Tomisaki, Mai and Oktaviani, Nurul Mutmainah Diah and Setyaningsih, Widiastuti and Einaga, Yasuaki and Amrillah, Tahta and Amalina, Ilma and Basirun, Wan Jeffrey and Anjani, Qonita Kurnia (2026) Electrochemical Sensor of Ciprofloxacin on Screen-Printed Electrode Modified with Boron-Doped Diamond Nanoparticles and Nickel Oxide Nanoparticles Biosynthesized Using Spatholobus littoralis Hassk. Root Extract. Biosensors, 16 (3).

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Abstract

Ciprofloxacin (CIP) is an antibiotic that is widely used in humans and animals. However, the compound has been detected in animal-derived products and the environment due to its extensive use, causing serious concern for public health and environmental safety. The issue raises the urgent need to develop innovative techniques to monitor CIP. Therefore, this study aims to develop a simple and sensitive CIP sensor called the boron-doped diamond nanoparticle-modified screen-printed electrode (BDD NPs/SPE) and the nickel oxide nanoparticle-modified BDD NPs/SPE (NiO NPs/BDD NPs/SPE). NiO NPs were synthesized via green synthesis using Spatholobus littoralis Hassk. root extract as the reducing agent. The formation and characteristics of NiO NPs were then confirmed through a UV-Vis spectrophotometer, XRD, PSA, FT-IR, and XPS. The successful modification of SPE was confirmed through SEM-EDX, followed by measurements using square-wave voltammetry. The results showed that the modified SPE could detect CIP over a concentration range of 0.1�100 µM and produced a low detection limit of 0.109 µM for BDD NPs/SPE and 0.054 µM for NiO NPs/BDD NPs/SPE. The proposed method was successfully applied to the determination of CIP in commercial tablets, milk, and human urine, with a satisfactory recovery from 95 to 100. The current study successfully developed a simple yet highly sensitive sensor that enabled robust, reliable, and efficient detection of CIP, showing its strong potential for practical applications. © 2026 by the authors.

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