RIA HANISTYA (2020) PENGARUH PENAMBAHAN SPACE PEPTIDE TERHADAP EFEKTIFJTAS, STABILITAS FISIK DAN IRITABILITAS SEDIAAN GEL FREEZE DRIED AMNIOTIC MEMBRANE STEM CELLMETABOLITE PRODUCT SEBAGAI ANTI AGING. Thesis thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Amniotic Membrane Stem Cells (AMSC) are bet1er treatment for antiaging in human skin because they are easy to produce and inexpensive. The metabolite product of AMSC contains several growth facto rs like TGF-~ and cytokines which significantly improve skin quality by increase proliferation and migration of derma l fibroblast as well collagen synthesis. However, growth factors are hydrophilic molecule with molecular size larger than 20 kDa therefore it cannot penetrate into stratum corneum to enhance it pharmacological effect. To enhance the skin penetration of AMSC the right gel formulation using enhancer SPACE-Peptide was made. Various kinds of SPACE-Peptide concentration were used to examine the effect of increasing SPACE-Peptide to effectivity, physical stability and irritability of gel freeze dried amniotic membrane metabolite product. The concentration between AMSC-MP:SPACE-Peptide varies from (1:0), (1: 1), ( 1:2), (1:3). pH value, spredabi lity test and visual observation including colour, odour, consistency and texture were conducted to determine the characterization of the gel. Gel freeze dried amniotic membrane metabolite product showed good organoleptic appearance and characteristic in all of formula. Effectivity test was determined by depth of penetration after 30, 60 and 120 minutes application of the gel using rhodamine B as fluorescent label using fluorescent microscopy. Antiaging activity after 20 days application of sample in mice was measured by scoring of collagen density and number of fibroblast in hispathological preparation in MassonTrichrome staining observed by light microscopy. The irritability test after 24 hour application of sample was conducted by scoring hispathology using Hematoxilin Eosin staining observed by light microscopy. The last, physical stability of each formula determined during 30 days storage in room temperature. The resu lt showed there were no significant difference in physical characteristic and stability between formula but there were significant difference in effectivity and irritability score. The formula with highest concentration of SPACE-Peptide (I :3) showed the highest score in antiaging activi ty but reach the highest score in irritability.

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