Yan Efrata Sembiring (2022) Penggunaan Arteri Mesentrika Bovine Yang Di-Seeding-Kan Dengan Endothelial Progenitor Cell (Epc) Dan Mesenchymal Stem Cell (Msc) Sebagai Prototype Pembuatan Graft Pembuluh Darah Diameter Kecil. Disertasi thesis, UNIVERSITAS AIRLANGGA,.

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Abstract

Background. A small-diameter vascular graft (<6 cm) is a gold standard for bypass coronary surgery and peripheral artery surgery but there is some limitation for this autologous graft. Tissue-engineered vascular graft (TEVG) is one of the promising solutions. Objective. To develop a small diameter vascular graft from the bovine mesenteric artery in combination with Endothelial Progenitor Cell (EPC) and Mesenchymal Stem Cell (MSC). Methods. The research was designed as an in vitro experiment, with a vascular scaffold as a subject. This research was conducted completely randomized and had several stages. First, the vascular scaffold was harvested from bovine vascular. Then, the seeding process was done with 2 stem cells (EPC and MSC). Lastly, each of the vascular implant benefits was analyzed. Results. Acellular vascular graft which had been seeded with MSC and EPC expressed CD90+ and CD34+, 7,67±2,260 and 8,50±2,492 respectively compared to acellular (control) 2,00±1,414 (p<0,05). Compared to the acellular group, there is a significantly higher expression of collagen type 1, collagen type 4, and elastin in both EPC and MSC. But no significant difference in both EPC and MSC (p<0,05). There is a significantly higher expression of eNOS in EPC (7.33±1.86) and MSC (5,17±1,169) (p<0,05) compared to the acellular group. From tensile strength and burst pressure examination, showed that EPC and MSC-seeded scaffolds are stronger than acellular counterparts (p<0,05). Conclusions. Acellular vascular graft which was seeded with EPC or MSC stem cells had the potency to be a small diameter tissue engineered vascular graft.

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