Arni Kusuma Dewi (2022) Analisis Efek Neuroprotektan Ekstrak Biji Klabet Pada Anak Tikus Model Autism Spectrum Disorder. Disertasi thesis, Universitas Airlangga.

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Abstract

Backgroud : Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder which had an increasing prevalence every year. Prenatal exposure to valproic acid (VPA) is a well-established pharmacological risk factor for ASD phenotypes. This study evaluated the neuroprotective potential of fenugreek (Trigonella foenumgraecum) seed extract in a prenatal VPA-induced rat model of ASD by mechanism of their antidiabetics. Method : Pregnant Wistar rats were allocated into five groups (n = 6 per group): control (saline), VPA, VPA + fenugreek extract 100 mg/kg, VPA + fenugreek extract 200 mg/kg, and VPA + fenugreek extract 300 mg/kg. VPA (400 mg/kg) was administered as a single oral dose on gestational day (GD) 15. Fenugreek extract was administered orally once daily from GD11–GD20. Male offspring were assessed for social test, histology changes, immunohistochemistry quantified for myelin basic protein (MBP), Glucose transporter 1 (GLUT 1) and ATP syntetase (ATPase). Result : The VPA group showed reduced social test and increased behavioural tes and decreased pattern of histology compared to control. Fenugreek extract at 200 mg/kg significantly prevent ASD by increased social test, decreased behavioural test also showed stronger restoration of histology pattern compared with the VPA group (p < 0.05). No difference intensity of GLUT 1 and ATPase. Conclusion : Fenugreek seed extract exhibits neuroprotective against prenatal VPA-induced ASD-like phenotypes, with dose of 200 mg/kg , but not through the antidiabetic pathway. Further studies are required to analyzing other mechanism pathways. Keywords | Fenugreek seed extract, Rat model Autism, neuroprotector, Good health and well-being.

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