RISKY VITRIA PRASETYO (2019) MEKANISME AKTIVASI DAN PROLIFERASI SEL PUNCA GINJAL OLEH LACTOBACILLUS PLANTARUM IS-10506 DALAM PERCEPATAN PERBAIKAN EPITEL TUBULUS GINJAL (Studi eksperimental pada kerusakan epitel tubulus ginjal tikus Sprague Dawley yang mendapatkan lipopolisakarida Escherichia coli ATCC® 25922™). Disertasi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

BACKGROUND Lactobacillus plantarum IS-10506 is a novel probiotic isolated from Indonesian traditional fermented buffalo milk called dadih. Probiotics are clinically proven to be effective in accelerating recovery after urinary tract infection (UTI) and in decreasing recurrent UTI in children with or without structural disorders of the urinary tract. OBJECTIVE This study aimed to investigate the role of the probiotic L. plantarum IS-10506 in activating and regenerating renal epithelial stem cells in pyelonephritic rats. METHODS Fifty five 20-weeks-old male Sprague Dawley rats were randomized into control, lipopolysaccharide (LPS) and treatment (LPS and probiotic) groups. Pyelonephritis was induced with Escherichia coli ATCC® 25922™ LPS (1.5×107 CFU) administered via a urethral catheter on the first experimental day to the LPS and treatment groups. Microencapsulated L. plantarum IS-10506 (1.43×109 CFU/day) was orally administered once daily for up to 14 days to the treatment group. On days 3, 5, 7, 10 and 14, five rats per group were sacrificed and their kidneys were immunohistochemically analyzed for expression of IL-10, lipocalin- 2 and Ki-67 in the renal tubular cells. RESULTS IL-10 expression was upregulated starting from day 3 through day 14 in the treatment group (P<0.05) compared with that in the control and LPS groups. Moreover, treatment with the probiotic led to increased activation of renal tubular stem cells, as indicated by a higher lipocalin-2 expression (P<0.05), and further proliferation of renal tubular stem cells was documented by a higher Ki-67 expression (P<0.05). CONCLUSION Taken together, these results indicate that the probiotic L. plantarum IS-10506 improves renal injury in pyelonephritic rats by activating endogenous renal tubular stem cells to proliferate into mature renal tubular epithelial cells.

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