Elizabeth Poedjiono, 021011151 (2013) EFEKTIVITAS EKSTRAK PROPOLIS 12,38% DAN CHLORHEXIDINE GLUCONATE 0,2% TERHADAP BIOFILM Porphyromonas gingivalis. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Many studies have reported the most common cause of persistent inflammation is bacterial biofilms. Bacteria growing in the biofilm can be doubled up to a thousand more resistant to antimicrobial agents than planktonic form of the same bacteria. One of the dominant bacteria on periapical tissue damage is Porphyromonas gingivalis. The irrigation solution that often used to remove the bacteria is Chlorhexidine gluconate. However, chlorhexidine can’t dissolve necrotic tissue remnants and damage the soft tissue if used excessively, so it needs an alternative material that can eliminate these side effects. Propolis extract is an alternative material that contains flavonoid, terpenoid, apigenin and other synergistic compound as an antibacterial. Purpose. To find out the efficacy of 12,38% propolis extract and Chlorhexidine gluconate 0,2% against Porphyromonas gingivalis biofilm Method. This research is a laboratory experimental with post test only control group design. Porphyromonas gingivalis ATCC 33277 was diluted according to Mc. Farland standard 106 CFU/mL in Tryptic Soy Broth (TSB) medium and inserted into microtitterplate flexible U bottom. Incubated for 6x24 hour and checked with a simple staining to see the formation of biofilm. Propolis extract and 0,2% Chlorhexidine gluconate was added and OD (optical density) readings done with a wavelength of 567 nm Result. Average OD value of Porphyromonas gingivalis biofilm after the addition of 12.38% propolis extract is 0.135 while after addition of 0.2% chlorhexidine gluconate is 0.481 Conclusion. 12.38% propolis extract has higher effectiveness than 0.2% chlorhexidine gluconate against Porphyromonas gingivalis biofilm.

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