REISKA KUMALA BAKTI, 020710006 (2010) DAYA ANTIBAKTERI INFUSUM KULIT BUAH DELIMA (Punica granatum L.) TERHADAP Streptococcus viridans SECARA in vitro. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Pulp diseases are caused by physic, chemical, and the most agent that is bacteria. The main microorganism in causing irreversible pulpitis and periapical disease is Streptococcus viridans. These bacteria infected the root canal, and an infected root canal need treatment. Success of root canal treatment is supported by an adequate root canal irrigation. The Punica granatum fructus cortex phytocemistrically contains alkaloids (pelletierine) and phenol, so that it has antimicrobial agent. Antimicrobial effect is one of the requirement as a good irrigation material. Purpose. The aim of the study was to determine the minimum concentration of Punica granatum fructus cortex infusion that has antibacterial potency against Streptococcus viridans. Method. This research was a laboratory experimental study. A serial dilution method was used to determine the minimum concentration of Punica granatum fructus cortex infusion that could kills Streptococcus viridans by colony counting bacteria in blood agar media. Growth of bacterial colonies in blood agar is calculated manually in colony forming unit (cfu). Result. There are decreasing numbers of Streptococcus viridans bacterial colonies in concentration 6.25%, 12.5% and 25% of infusion, compared by positive control group with significant differences in each study group (p<0.05). At the concentration of 25% was not revealed any Streptococcus viridans growth. Conclusion. The minimum antibacterial concentration of infusion againts S. viridans growth is 25%.

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