Dian Meiria Nurhayati, 020610089 (2011) KONSENTRASI EFEKTIF EKSTRAK DAUN SALAM DALAM MENGHAMBAT PERTUMBUHAN STREPTOCOCCUS MUTANS PADA POLYVINYL SILOXANE. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Polyvinyl siloxane impression materials applications in a variety of indirect procedures in prosthodontics and restorative dentistry. Favourable handling properties, good patient acceptance and exellent physical properties resulted in its popularity in todays practice. Transmission of the oral cavity bacteria (Streptococcus mutans) can occur during the making of impression in the patient's mouth. Oral cavity microorganisms can be brought on by making the impression because of the adsorption of microorganisms on the surface of an impression. To prevent transmission of bacteria, polyvinyl siloxane impression needs to be disinfected. Purpose. The aim of this research is to find out the effective concentration of Bay leaf extract (Eugenia polyantha wights) in inhibiting the multiplication of Streptococcus mutans on polyvinyl siloxane impression. Method. Steril polyvinyl siloxane impression puts into test tubes containing a suspension of Streptococcus mutans, then incubated for 24 hours at 37 ° C. Soaking for 10 minutes done in regards to the concentration of Bay Leaf extract respectively 50%, 25%, 12.5%, and 6.25% and the control (sterile distilled water). Then inserted into the media (BHIB) and vibrated for 30 seconds. Then do the seeding of 0.1 ml suspension of Streptococcus mutans from BHIB on TYC media, incubation for 48 hours at 37 ° C, and count the number of Streptococcus mutans. Result. There are significant differences of Streptococcus mutans colonies embedded in polyvinyl siloxane after being soaked in sterile distilled water as a control and after incubating the Bay Leaf extract (Eugenia polyantha wights) with a concentration of 50%, 25%, 12.5%, and 6.25%. Conclusion. The higher concentration of Bay Leaf extract (Eugenia polyantha wights), the less the number of colonies of Streptococcus mutans.

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