NATALYA TANRI SUDARNO, 020313194 (2007) UJI SITOTOKSISITAS MINYAK BUAH MERAH TERHADAP SEL FIBROBLAS DENGAN ESAI MTT. Skripsi thesis, UNIVERSITAS AIRLANGGA.
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Abstract
The requirements of dental material include not toxic, not irritant, no carcinogenic potential, nor cause an allergic response with the use in oral cavity. The use of Pandanus conoideus Lam is not truly safe as an alternatives drug, because drug can be poisonous at certain condition. The purpose of this study was to know the cytotoxicity of Pandanus conoideus Lam with the different variation of concentration of Pandanus conoideus Lam using MTT assay. MTT assay based on the cellular conversion of 3-(4, 5-dimethylthiazol-2 yl)-2, 5-di phenyl¬tetrazolium bromide (M7T) into purple formazan crystals by mitochondria) enzymes of metabolically active cells. This formazan product was easily detected by using the cellular conversion sphectrophotometer. Analysis was done statistically using one way ANOVA and continued by HSD. The result showed that the increasing concentration of Pandanus conoideus Lam extract solution i. e 50%, 60%, 70%, 80%, 90% would not increase cytotoxycity, and the percentage of active BHK-21 (Baby Hamster Kidney) cell lines on these concentration remaines more than 88%.
Item Type: | Thesis (Skripsi) | |||||||||
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Additional Information: | KKA KK KG.75/07 Sud u | |||||||||
Uncontrolled Keywords: | MTT assay, cytotoxycity, Pandanus conoideus Lam | |||||||||
Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QK Botany > QK1-989 Botany |
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Divisions: | 02. Fakultas Kedokteran Gigi | |||||||||
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Depositing User: | Nn Dhani Karolyn Putri | |||||||||
Date Deposited: | 25 Aug 2007 12:00 | |||||||||
Last Modified: | 29 Sep 2016 15:11 | |||||||||
URI: | http://repository.unair.ac.id/id/eprint/19692 | |||||||||
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