FARADIAH HAYATI, 021011043 (2014) UJI TOKSISITAS HIDROGEN PEROKSIDA 3%, SODIUM HYPOCHLORITE 2,5%, DAN CHLORHEXIDINE GLUCONATE 0,2% TERHADAP SEL FIBROBLAS BHK-21 : Penelitian Eksperimental Laboratoris. Skripsi thesis, UNIVERSITAS AIRLANGGA.

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Abstract

Background. Hydrogen Peroxide 3%, Sodium Hypochlorite 2,5%, and Chlorhexidine Gluconate 0,2% are some dental liquid that is often used as a root canal irrigation. That liquid should not be cytotoxic. Purpose. The aim of this study to prove the toxicity effect of Hydrogen Peroxide 3%, Sodium Hypochlorite 2,5%, and Chlorhexidine Gluconate 0,2% on BHK-21 Fibroblast Cell with MTT Assay. Methode. Hydrogen Peroxide 3%, Sodium Hypochlorite 2,5%, and Chlorhexidine Gluconate 0,2% are applied on BHK-21 Fibroblast Cell. Culture BHK-21 Fibroblast Cell with MTT Assay is incubated at 37 °C for 24 hours. ELISA Reader used to read the result of the experimental study. The data is compared and examined by One Way ANNOVA and HSD Turkey Test. Result. Hydrogen Peroxide 3% is the most toxic to the fibroblast cells followed by Hypochlorite 2,5%, and Chlorhexidine Gluconate 0,2%. Conclusion. Hydrogen Peroxide 3%, Sodium Hypochlorite 2,5%, and Chlorhexidine Gluconate 0,2% toxic to BHK-21 Fibroblast Cell.

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